Active execution canvas
Not a static filing notebook. Discord-style logs, live step timers, and a bench prompt bar that scales and troubleshoots as you pipet.
Private beta · protocol libraries indexing
AI lab assistant for scientists by scientists
ProtocolOS is an active execution assistant — not a static filing notebook. Real-time bench execution, dynamic media-prep scaling, and instant troubleshooting live on the step you are running.
Built for biomedical researchers · Paid plans, SOC 2, and lab sharing coming soon
Modular + Add Tool
Drop a countdown timer, dynamic media-prep card, or dilution calculator onto any step — only when that run needs it.
Dynamic media prep
Change the batch volume and ingredient masses recalc instantly — LB broth, agar recipes, and master-mix overage.
Instant troubleshooting
Photograph a blot, gel, or instrument screen in the Live Demo. A dedicated workspace diagnostics copilot is coming soon.
protocolos.ai/notebook
Step 2 · Assemble 20 µL reactions
Scale for 24 wells at +10% overage. Confirm water volume before plating.
Countdown timer
8:00 remaining · 37 °C hold
Add Tool
Capabilities
Built for the failure modes that actually cost you a week
Not a chatbot bolted onto a wiki. ProtocolOS is an active execution assistant — protocols, reagents, instruments and diagnostics as first-class objects.
Grounded troubleshooting at the bench
Coming SoonDiagnoses failures against your lab's exact SOPs and vendor manuals. Available in the Live Demo today; the dedicated workspace copilot is coming soon.
“My ligation keeps failing at 4:1 insert:vector.”
Your SOP specifies T4 ligase at 16 °C overnight, but the last three runs ran at room temperature for 20 min. Ligase efficiency on blunt ends drops sharply above 20 °C.
SOP-CLONE-003 §4.1NEB M0202 manual p. 7
Multimodal bench vision
Coming SoonSnap photos of gels, colony plates or hardware screens in the Live Demo. The workspace Diagnostics module is coming soon.
Protocol DAG & git-like diffs
Visually track what changed between successful and failed runs: pH, lot numbers, temperatures, incubation times.
Cold-chain & decay tracking
Coming SoonAutomated freeze–thaw and expiration monitoring for sensitive antibodies and enzymes. Coming soon.
Institutional lab memory
Coming SoonShared troubleshooting knowledge that survives trainee turnover. Local run memory exists; lab-wide memory is coming soon.
STAR Methods & publication generator
Coming SoonJournal-ready methods with verified RRIDs and the parameters you actually ran. Notebook can export PDF / Markdown today; STAR Methods compile is coming soon.
star_methods.md
4 RRIDs verified · 0 unsourced claims
- Reagent
- Q5 High-Fidelity DNA Polymerase (NEB, M0491L, lot 10221934)
- RRID
- SCR_020542 — verified against SciCrunch, 2026-08-19
- Cycling
- 98 °C 30 s; 35 × (98 °C 10 s, 62 °C 20 s, 72 °C 45 s); 72 °C 2 min
- Deviation
- Anneal raised 57.5 → 60.0 °C after run #47 primer-dimer